pAND_Int2
(Plasmid #60590)

• Purpose: A transcriptional AND circuit constructed by layering NOR and NOT gates
• Depositing Lab: Christopher Voigt
• Publication: Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 2014 Oct 26.

Backbone

• Vector backbone: unknown
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: AND_Int2
• Alt name: Integrase2
• Species: Synthetic
• Promoter: PPhlF

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: unknown

Resource Information

• Supplemental Documents:
  • pAND_Int2

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The AND function is composed of two NOT gates and a NOR gate using the SrpR, BM3R1, and PhlF repressors, respectively. Each repressor contains a variant of the RiboJ insulator (SccJ, SarJ, and LtsvJ) to reduce the effect of genetic context that arises from different promoter inputs. The output promoter of the AND circuit (PPhlF) is connected to the Int2 integrase, which then interacts with the array to permanently record whether both input signals (aTc and IPTG) were observed at the same time.