pMemoryArray
(Plasmid
#60585)
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PurposeAn array constructed by concatenating the attB/P sites of the 11 phage integrases and can be used to distinguish 2048 possible combinations of events.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 60585 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneunknown
- Total vector size (bp) 3793
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH10B
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameMemoryArray
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Alt name11 attB/P sites and spacers
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SpeciesSynthetic
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CAT-R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
An array was constructed by concatenating the attB/P sites of the 11 phage integrases to form a linear 2kb piece of DNA. Random spacers (50 bp) with 50% GC content are included between the att sites.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMemoryArray was a gift from Christopher Voigt (Addgene plasmid # 60585 ; http://n2t.net/addgene:60585 ; RRID:Addgene_60585) -
For your References section:
Permanent genetic memory with >1-byte capacity. Yang L, Nielsen AA, Fernandez-Rodriguez J, McClune CJ, Laub MT, Lu TK, Voigt CA. Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 2014 Oct 26. 10.1038/nmeth.3147 PubMed 25344638