pIntegrase_5
(Plasmid
#60577)
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Purposeplasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 60577 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneunknown
- Total vector size (bp) 6207
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH10B
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameIntegrase_5
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SpeciesStreptomyces phage PhiK38-1
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MutationCodon optimized for E.coli
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GenBank IDBAF03598
- Promoter pBAD
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer pBAD-F (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pIntegrase_5 was a gift from Christopher Voigt (Addgene plasmid # 60577 ; http://n2t.net/addgene:60577 ; RRID:Addgene_60577) -
For your References section:
Permanent genetic memory with >1-byte capacity. Yang L, Nielsen AA, Fernandez-Rodriguez J, McClune CJ, Laub MT, Lu TK, Voigt CA. Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 2014 Oct 26. 10.1038/nmeth.3147 PubMed 25344638
Map uploaded by the depositor.
