pmTurquoise-C1
(Plasmid
#60558)
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PurposeExpresses the cyan fluorescent protein variant mTurquoise
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 60558 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-C1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4000
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemTurquoise
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SpeciesSynthetic
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Insert Size (bp)720
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI (not destroyed)
- 3′ cloning site BsrGI (not destroyed)
- 5′ sequencing primer CMV (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pmTurquoise-C1 was a gift from Dorus Gadella (Addgene plasmid # 60558 ; http://n2t.net/addgene:60558 ; RRID:Addgene_60558) -
For your References section:
Bright cyan fluorescent protein variants identified by fluorescence lifetime screening. Goedhart J, van Weeren L, Hink MA, Vischer NO, Jalink K, Gadella TW Jr. Nat Methods. 2010 Feb;7(2):137-9. doi: 10.1038/nmeth.1415. Epub 2010 Jan 17. 10.1038/nmeth.1415 PubMed 20081836