pDEST-hPDIb′a′
(Plasmid
#60556)
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Purpose(Empty Backbone) enhance solubility and correct disulfide bond-formation of fused protein expressed in E.coli
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 60556 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepDEST-0GWA
- Backbone size (bp) 7105
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Modifications to backboneinsert His6-hPDIb'a' tag
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Vector typeBacterial Expression
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Tag
/ Fusion Protein
- His6-hPDIb'a' tag (N terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DB3.1
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Growth instructionsMust be grown in a strain resistant to ccdB
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Copy numberUnknown
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDEST-hPDIb′a′ was a gift from Han Choe (Addgene plasmid # 60556 ; http://n2t.net/addgene:60556 ; RRID:Addgene_60556) -
For your References section:
Expression and purification of biologically active human FGF2 containing the b'a' domains of human PDI in Escherichia coli. Song JA, Koo BK, Chong SH, Kwak J, Ryu HB, Nguyen MT, Vu TT, Jeong B, Kim SW, Choe H. Appl Biochem Biotechnol. 2013 May;170(1):67-80. doi: 10.1007/s12010-013-0140-3. Epub 2013 Mar 8. 10.1007/s12010-013-0140-3 PubMed 23471584
Map uploaded by the depositor.
