pcDNA3.1-CibN-dCas9
(Plasmid #60551)

• Purpose: Expresses CibN-dCas9 in mammalian cells
• Depositing Lab: Charles Gersbach
• Publication: Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/nchembio.1753.

Backbone

• Vector backbone: pcDNA3.1
• Backbone size w/o insert (bp): 5333
• Total vector size (bp): 10118
• Vector type: Mammalian Expression, CRISPR
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CibN-dCas9
• Species: A. thaliana (mustard weed); Streptococcus pyogenes
• Insert Size (bp): 4785
• Mutation: inactivating Cas9 mutations D10A and H840A
• Promoter: CMV
• Tags / Fusion Proteins:
  • FLAG (N terminal on insert)
  • SV40 NLS (N terminal on insert)
  • SV40 NLS (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: ATTGACGCAAATGGGCGGTAGGCGTGT
• 3′ sequencing primer: GCC TGC TAT TGT CTT CCC AAT

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry