Skip to main content
Addgene

pENTR-D-TOPO-PAmCherry1_160-236-MCS
(Plasmid #60546)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 60546 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pENTR/D-TOPO
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2580
  • Total vector size (bp) 2883
  • Modifications to backbone
    Added flanking MCSs
  • Vector type
    Cloning

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PAmCherry1 C-terminal fragment
  • Alt name
    RC160-236
  • Insert Size (bp)
    234
  • Mutation
    amino acids 160-236

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer M13 forward
  • 3′ sequencing primer M13 reverse
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Parent plasmid (full-length PAmCherry1 in pENTR) (Addgene plasmid 60608) originally reported in Nan et al., 2013 (PMID:24158481).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENTR-D-TOPO-PAmCherry1_160-236-MCS was a gift from Xiaolin Nan (Addgene plasmid # 60546 ; http://n2t.net/addgene:60546 ; RRID:Addgene_60546)

  • For your References section:

    Photoactivated localization microscopy with bimolecular fluorescence complementation (BiFC-PALM) for nanoscale imaging of protein-protein interactions in cells. Nickerson A, Huang T, Lin LJ, Nan X. PLoS One. 2014 Jun 25;9(6):e100589. doi: 10.1371/journal.pone.0100589. eCollection 2014. 10.1371/journal.pone.0100589 PubMed 24963703
Related items:
Commonly requested with: