pENTR-D-TOPO-PAmCherry1_1-159-MCS
(Plasmid #60545)

• Purpose: Cloning PAmCherry1 N-terminal fragment (RN) for BiFC-PALM
• Depositing Lab: Xiaolin Nan
• Publication: Nickerson et al PLoS One. 2014 Jun 25;9(6):e100589. doi: 10.1371/journal.pone.0100589. eCollection 2014.

Backbone

• Vector backbone: pENTR/D-TOPO
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 2580
• Total vector size (bp): 3090
• Modifications to backbone: Added MCS. C-terminal MCS was lost during cloning.
• Vector type: Cloning

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PAmCherry1 N-terminal fragment
• Alt name: RN1-159
• Insert Size (bp): 477
• Mutation: amino acids 1-159

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: M13 forward
• 3′ sequencing primer: M13 reverse

Resource Information

• Supplemental Documents:
  • GenBank

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Parent plasmid (full-length PAmCherry1 in pENTR) (Addgene plasmid 60608) originally reported in Nan et al., 2013 (PMID:24158481).