AA0250-mCherry-2a-hM4D-nrxn1a rev
(Plasmid #60544)

• Purpose: Co-expresses hM4Dnrxn and a red fluorescent label from a Cre-dependent virus
• Depositing Lab: Scott Sternson
• Publication: Stachniak et al Neuron. 2014 May 21;82(4):797-808. doi: 10.1016/j.neuron.2014.04.008. Epub 2014 Apr 24.

Backbone

• Vector backbone: AAV2
• Backbone size w/o insert (bp): 5048
• Total vector size (bp): 7478
• Vector type: AAV, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL10 Gold
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: mCherry
• Insert Size (bp): 710
• Promoter: CAG

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: SpeI (not destroyed)
• 3′ cloning site: SpeI (not destroyed)
• 5′ sequencing primer: pCAG-F
• 3′ sequencing primer: WPRE-R

Gene/Insert 2

• Gene/Insert name: muscarinic receptor 4, axon targeted variant
• Alt name: M4R
• Species: H. sapiens (human)
• Insert Size (bp): 1720
• Entrez Gene: CHRM4 (a.k.a. HM4, M4R)
• Promoter: CAG
• Tag / Fusion Protein:
  • 2xHA (C terminal on insert)

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: pCAG-F
• 3′ sequencing primer: WPRE-R

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• A portion of this plasmid was derived from a plasmid made by: Bryan Roth, UNC
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry