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Addgene

pDONR223 PAK5 T538N
(Plasmid #60537)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 60537 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDONR223
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5005
  • Vector type
    Gateway Donor vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Pak5
  • Alt name
    Pak7
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2160
  • Mutation
    T538N
  • Entrez Gene
    PAK5 (a.k.a. PAK7)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDONR223 PAK5 T538N was a gift from John Brognard (Addgene plasmid # 60537 ; http://n2t.net/addgene:60537 ; RRID:Addgene_60537)

  • For your References section:

    Targeted genetic dependency screen facilitates identification of actionable mutations in FGFR4, MAP3K9, and PAK5 in lung cancer. Fawdar S, Trotter EW, Li Y, Stephenson NL, Hanke F, Marusiak AA, Edwards ZC, Ientile S, Waszkowycz B, Miller CJ, Brognard J. Proc Natl Acad Sci U S A. 2013 Jul 23;110(30):12426-31. doi: 10.1073/pnas.1305207110. Epub 2013 Jul 8. 10.1073/pnas.1305207110 PubMed 23836671
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