3xNLS-SGFP2(T65G)-T2A-SGFP2-3xNES
(Plasmid #60492)

• Purpose: Co-expresses two green fluorescent protein variants, a short fluorescence lifetime GFP variant in the nucleus and a GFP variant with a long lifetime in the cytoplasm
• Depositing Lab: Dorus Gadella
• Publication: Joosen et al J Microsc. 2014 Sep 1. doi: 10.1111/jmi.12168.

Backbone

• Vector backbone: pSGFP2-C1
• Total vector size (bp): 5726
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: 3xnls-SGFP2(T65G)
• Species: Synthetic
• Insert Size (bp): 816
• GenBank ID:
• Promoter: CMV
• Tag / Fusion Protein:
  • SGFP2(T65G) (C terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: AGGTCTATATAAGCAGAGC

Gene/Insert 2

• Gene/Insert name: SGFP2-3xNES
• Species: Synthetic
• Insert Size (bp): 855
• GenBank ID:
• Promoter: CMV
• Tag / Fusion Protein:
  • SGFP2 (N terminal on insert)

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: AGGTCTATATAAGCAGAGC
• 3′ sequencing primer: TCTACAAATGTGGTATGGC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry