pH2B-SCFP1-2A-mTq2-NES
(Plasmid #60491)

• Purpose: Co-expresses two cyan fluorescent protein variants, a short fluorescence lifetime CFP variant in the nucleus and a CFP variant with a long lifetime (mTurquoise2) in the cytoplasm
• Depositing Lab: Dorus Gadella
• Publication: Joosen et al J Microsc. 2014 Sep 1. doi: 10.1111/jmi.12168.

Backbone

• Vector backbone: pmTurquoise2-C1
• Total vector size (bp): 5950
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: H2B-SCFP1
• Alt name: Histone 2B
• Species: Synthetic
• Insert Size (bp): 1113
• GenBank ID:
• Promoter: CMV
• Tag / Fusion Protein:
  • SCFP1 (C terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: AGGTCTATATAAGCAGAGC

Gene/Insert 2

• Gene/Insert name: mTurquoise2-NES
• Species: Synthetic
• Insert Size (bp): 765
• GenBank ID:
• Tag / Fusion Protein:
  • mTurquoise2 (N terminal on insert)

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: AGGTCTATATAAGCAGAGC
• 3′ sequencing primer: TCTACAAATGTGGTATGGC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry