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Purpose(Empty Backbone) This plasmids is a modular mini-Tn5 vector that could be used to generate random mutagenesis libraries or to insert heterologous DNA into a target genome
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 60487 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBAM1
- Backbone size (bp) 4384
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin and Kanamycin, 100 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Pir1
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Growth instructionsPlease test 2-3 colonies before storing a glycerol stock.
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Copy numberLow Copy
Cloning Information
- Cloning method Restriction Enzyme
Resource Information
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Addgene Notes
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBAM1 was a gift from Víctor de Lorenzo (Addgene plasmid # 60487 ; http://n2t.net/addgene:60487 ; RRID:Addgene_60487) -
For your References section:
pBAM1: an all-synthetic genetic tool for analysis and construction of complex bacterial phenotypes. Martinez-Garcia E, Calles B, Arevalo-Rodriguez M, de Lorenzo V. BMC Microbiol. 2011 Feb 22;11:38. doi: 10.1186/1471-2180-11-38. 10.1186/1471-2180-11-38 PubMed 21342504
Map uploaded by the depositor.
