pSynSRE-T-Luc
(Plasmid #60444)

• Purpose: Uses the hamster HMG-CoA synthase promoter sequence (-324/-225) and minimal HMG-CoA sythnase TATA box (-28/+39) to study SREBP-dependent transcriptional activation.
• Depositing Lab: Timothy Osborne
• Publication: Dooley et al J Biol Chem. 1998 Jan 16;273(3):1349-56.

Backbone

• Vector backbone: pGL2 basic
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 5357
• Total vector size (bp): 5504
• Modifications to backbone: Contains the hamster HMG-CoA synthase promoter sequence harboring SREBP responsive region (-324/-225) fused directly to minimal HMG-CoA sythase TATA box (-28/+39) that is linked to luciferase in polylinker of pGL2 basic
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HMG-CoA synthase promoter (-324/-225)
• Alt name: 3-Hydroxy-3-methylglutaryl-coenzyme A synthase promoter
• Species: Hamster
• Insert Size (bp): 186
• Promoter: HMG-CoA synthase (-324/-225) & TATAA (-28/+39)
• Tag / Fusion Protein:
  • Luciferase (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SstI or SacI (not destroyed)
• 3′ cloning site: HindIII for promoter and TATAA; NheI for promoter region only (not destroyed)
• 5′ sequencing primer: GLprimer1
• 3′ sequencing primer: GLprimer2

Resource Information

• Articles Citing this Plasmid:
  • 9 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry