UbcH5B
(Plasmid
#60443)
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PurposeExpress E2 UbcH5B in E. Coli
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 60443 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonePETSUMO
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Backbone manufacturerNovagen, modified
- Backbone size w/o insert (bp) 6000
- Total vector size (bp) 6400
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Modifications to backboneSUMO tag was added to the PET3a
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL1 Blue
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameUbcH5B
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SpeciesH. sapiens (human)
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Entrez GeneUBE2D2 (a.k.a. E2(17)KB2, PUBC1, UBC4, UBC4/5, UBCH4, UBCH5B)
- Promoter T7
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7 term (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
UbcH5B was a gift from Cynthia Wolberger (Addgene plasmid # 60443 ; http://n2t.net/addgene:60443 ; RRID:Addgene_60443) -
For your References section:
E2 ubiquitin-conjugating enzymes regulate the deubiquitinating activity of OTUB1. Wiener R, DiBello AT, Lombardi PM, Guzzo CM, Zhang X, Matunis MJ, Wolberger C. Nat Struct Mol Biol. 2013 Sep;20(9):1033-9. doi: 10.1038/nsmb.2655. Epub 2013 Aug 18. 10.1038/nsmb.2655 PubMed 23955022