Skip to main content
Addgene

AAVS1-SA-2A-NEO-CAG-RTTA3
(Plasmid #60431)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 60431 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    N/A
  • Vector type
    ZFN donor
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    rtTA3
  • Insert Size (bp)
    708
  • Promoter CAG

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer aacaacgccaagtcatac
  • 3′ sequencing primer ggagaagccttgctgaca
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAVS1-SA-2A-NEO-CAG-RTTA3 was a gift from Paul Gadue (Addgene plasmid # 60431 ; http://n2t.net/addgene:60431 ; RRID:Addgene_60431)

  • For your References section:

    A Doxycycline-Inducible System for Genetic Correction of iPSC Disease Models. Sim X, Cardenas-Diaz FL, French DL, Gadue P. Methods Mol Biol. 2015 Jan 29. 10.1007/7651_2014_179 PubMed 25630922
Related items:
Commonly requested with: