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pJAF205
(Plasmid #60407)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 60407 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p3XFLAG-myc-CMV-26
  • Backbone manufacturer
    Sigma
  • Modifications to backbone
    HindIII site was filled in to shift reading frame.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GMAP210
  • Alt name
    Trip11
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    5931
  • Entrez Gene
    Trip11 (a.k.a. 2610511G22Rik, 3110031G15Rik, 6030460N08Rik, AI450776, GMAP-210, TRIP230)
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Kpn1 (added by PCR) (unknown if destroyed)
  • 3′ cloning site Kpn1 (added by PCR) (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer hGH-pA-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJAF205 was a gift from Gregory Pazour (Addgene plasmid # 60407 ; http://n2t.net/addgene:60407 ; RRID:Addgene_60407)

  • For your References section:

    The Golgin GMAP210/TRIP11 anchors IFT20 to the Golgi complex. Follit JA, San Agustin JT, Xu F, Jonassen JA, Samtani R, Lo CW, Pazour GJ. PLoS Genet. 2008 Dec;4(12):e1000315. doi: 10.1371/journal.pgen.1000315. Epub 2008 Dec 26. 10.1371/journal.pgen.1000315 PubMed 19112494
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