pICE-NLS-mCherry
(Plasmid #60364)

• Purpose: Plasmid for expression of NLS-mCherry (control plasmid). Confers resistance to Puromycin. Expression is inducible in T-REx cells.
• Depositing Labs: Sébastien Britton, Patrick Calsou
• Publication: Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):9047-62. doi: 10.1093/nar/gku601. Epub 2014 Jul 16.

Backbone

• Vector backbone: pICE
• Backbone size w/o insert (bp): 5044
• Total vector size (bp): 5758
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NLS-mCherry
• Species: Synthetic
• Insert Size (bp): 766
• Promoter: CMV-tet
• Tags / Fusion Proteins:
  • NLS from SV40 T antigen (N terminal on backbone)
  • mCherry (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMVpromoter forward
• 3′ sequencing primer: BGH Reverse

Resource Information

• Supplemental Documents:
  • Author annotated GenBank sequence
• Addgene Notes:
  • Addgene Diagnostic Digest
• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry