pEGFP-N1-FUS/TLS-FLAGC
(Plasmid #60362)

• Purpose: Plasmid for expression of FLAG-GFP tagged human FUS/TLS (C-terminal tag). Confers resistance to G418.
• Depositing Labs: Sébastien Britton, Patrick Calsou
• Publication: Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):9047-62. doi: 10.1093/nar/gku601. Epub 2014 Jul 16.

Backbone

• Vector backbone: pEGFP-N1-FLAG
• Backbone size w/o insert (bp): 4777
• Total vector size (bp): 6351
• Modifications to backbone: Insertion of a FLAG tag in front of GFP together with an ATG and a strong kozak sequence.
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: FUS
• Alt name: TLS
• Alt name: POMP75
• Alt name: HNRNPP2
• Species: H. sapiens (human)
• Insert Size (bp): 1586
• GenBank ID: NM_004960.3
• Entrez Gene: FUS (a.k.a. ALS6, ETM4, FUS1, HNRNPP2, POMP75, TLS, altFUS)
• Promoter: CMV
• Tags / Fusion Proteins:
  • FLAG (C terminal on insert)
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: MluI (not destroyed)
• 5′ sequencing primer: CMVpromoter forward
• 3′ sequencing primer: EGFP-N

Resource Information

• Supplemental Documents:
  • Author annotated GenBank sequence
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry