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Addgene

pET-22HT-MSI1(7-192)R53E/R61E
(Plasmid #60354)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 60354 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-22b
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Total vector size (bp) 6000
  • Modifications to backbone
    pelB leader sequence was replaced with a His6-Gly tag followed by a TEV protease site. See pET-22HT-MSI1 (7–192) Addgene plasmid # 60245
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mouse MSI1 (7-192)
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    573
  • Mutation
    R53E and R61E mutations; contains amino acids 7-192 of Msi1
  • GenBank ID
    NM_008629.1
  • Entrez Gene
    Msi1 (a.k.a. Msi1h, Musahi1, m-Msi-1)
  • Promoter T7
  • Tags / Fusion Proteins
    • His (N terminal on backbone)
    • TEV protease site (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7-term
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-22HT-MSI1(7-192)R53E/R61E was a gift from Sean Ryder (Addgene plasmid # 60354 ; http://n2t.net/addgene:60354 ; RRID:Addgene_60354)

  • For your References section:

    Allosteric inhibition of a stem cell RNA-binding protein by an intermediary metabolite. Clingman CC, Deveau LM, Hay SA, Genga RM, Shandilya SM, Massi F, Ryder SP. Elife. 2014 Jun 16:e02848. doi: 10.7554/eLife.02848. 10.7554/eLife.02848 PubMed 24935936
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