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FUW-TetO-Lin28
(Plasmid #60345)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 60345 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    FUW-TetO
  • Backbone size w/o insert (bp) 8339
  • Total vector size (bp) 8969
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Lin28
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    630
  • Entrez Gene
    Lin28a (a.k.a. Gm10299, Lin-28, Lin28, Tex17, lin-28A)
  • Promoter Minimal CMV + TetO

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CGC CTG GAG ACG CCA TCC ACG CTG
  • 3′ sequencing primer AGA ATA CCA GTC AAT CTT TCA C
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FUW-TetO-Lin28 was a gift from Rudolf Jaenisch (Addgene plasmid # 60345 ; http://n2t.net/addgene:60345 ; RRID:Addgene_60345)

  • For your References section:

    Single-Cell Expression Analyses during Cellular Reprogramming Reveal an Early Stochastic and a Late Hierarchic Phase. Buganim Y, Faddah DA, Cheng AW, Itskovich E, Markoulaki S, Ganz K, Klemm SL, van Oudenaarden A, Jaenisch R. Cell. 2012 Sep 14;150(6):1209-22. doi: 10.1016/j.cell.2012.08.023. 10.1016/j.cell.2012.08.023 PubMed 22980981
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