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Purpose(Empty Backbone) This is a BAC vector that contains both wt and mutant loxP sites.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 60342 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBeloBAC11
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Backbone manufacturerMel Simon lab
- Backbone size (bp) 7507
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Modifications to backboneThe 2272 mutant loxP site was inserted immediately next to the NotI site upstream of the Lac Z gene. pBeloBac11 was obtained from New England BioLabs.
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Vector typeBacterial Expression, Cre/Lox
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH10B
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Copy numberLow Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
GenBank Accession # U51113 for vector backbone pBeloBAC11
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBeloBac11loxP2272 was a gift from Jon Coren (Addgene plasmid # 60342 ; http://n2t.net/addgene:60342 ; RRID:Addgene_60342) -
For your References section:
Retrofitting the BAC cloning vector pBeloBAC11 by the insertion of a mutant loxP site. Coren JS. BMC Res Notes. 2017 Jul 28;10(1):344. doi: 10.1186/s13104-017-2631-8. 10.1186/s13104-017-2631-8 [pii] PubMed 28754135
