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PGAL
(Plasmid #60337)

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Item Catalog # Description Quantity Price (USD)
Plasmid 60337 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    p416
  • Backbone size w/o insert (bp) 6000
  • Vector type
    Yeast Expression, Synthetic Biology ; Yeast synthetic hybrid promoter construct
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    promoter
  • Species
    S. cerevisiae (budding yeast)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site pmei (not destroyed)
  • 3′ cloning site xbai (not destroyed)
  • 5′ sequencing primer agt
  • 3′ sequencing primer agt
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    yECitrine

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PGAL was a gift from Hal Alper (Addgene plasmid # 60337 ; http://n2t.net/addgene:60337 ; RRID:Addgene_60337)

  • For your References section:

    Controlling promoter strength and regulation in Saccharomyces cerevisiae using synthetic hybrid promoters. Blazeck J, Garg R, Reed B, Alper HS. Biotechnol Bioeng. 2012 Nov;109(11):2884-95. doi: 10.1002/bit.24552. Epub 2012 May 17. 10.1002/bit.24552 PubMed 22565375
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