Myopathy Loop Mutant Nuclear Myosin I YFP
(Plasmid
#60243)
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PurposeExpresses a myopathy loop mutant (6 mutations) of the nuclear isoform of myosin IC attached to YFP and an NLS, which has impaired actin binding activity
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 60243 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEYFP-N1
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Backbone manufacturerClonetech
- Backbone size w/o insert (bp) 4700
- Total vector size (bp) 7923
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNuclear Myosin 1
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Alt namenuclear myosin 1B
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Alt nameUnconventional myosin-Ic
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SpeciesM. musculus (mouse)
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MutationDeleted I339, I340, G343, E344, E345,L346
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GenBank IDNM_001080774.1
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Entrez GeneMyo1c (a.k.a. MMIb, MYO1E, NMI, mm1beta, myr2)
- Promoter CMV
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Tags
/ Fusion Proteins
- EYFP (C terminal on insert)
- NLS (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site ECORI (not destroyed)
- 3′ cloning site ECORI (not destroyed)
- 5′ sequencing primer Unknown
- 3′ sequencing primer Unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Myopathy Loop Mutant Nuclear Myosin I YFP was a gift from Primal de Lanerolle (Addgene plasmid # 60243 ; http://n2t.net/addgene:60243 ; RRID:Addgene_60243) -
For your References section:
Long-range directional movement of an interphase chromosome site. Chuang CH, Carpenter AE, Fuchsova B, Johnson T, de Lanerolle P, Belmont AS. Curr Biol. 2006 Apr 18;16(8):825-31. 10.1016/j.cub.2006.03.059 PubMed 16631592
Map uploaded by the depositor.