pCRU5-inGFPt
(Plasmid #60236)

• Purpose: HTLV-1 based transfer vector encoding gfp-turbo reporter gene interrupted by intron
• Depositing Lab: Dmitriy Mazurov
• Publication: Shunaeva et al J Virol. 2015 Oct 15;89(20):10591-601. doi: 10.1128/JVI.01940-15. Epub 2015 Aug 12.

Backbone

• Vector backbone: pUCHR
• Backbone size w/o insert (bp): 6419
• Total vector size (bp): 8031
• Vector type: Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl2
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: inGFPt
• Insert Size (bp): 1612

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Mlu I (not destroyed)
• 3′ cloning site: Bgl II (not destroyed)
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Resource Information

• Supplemental Documents:
  • Protocol for generation of HIV-1 and HTLV-1 inLuc vectors with mR-int330 shRNA modification.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry