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pSLIK-Hygro-Myc-hAgo2
(Plasmid #60234)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 60234 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSLIK-Hygro
  • Backbone size w/o insert (bp) 13918
  • Total vector size (bp) 16500
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Argonaute2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2600
  • Entrez Gene
    AGO2 (a.k.a. CASC7, EIF2C2, LESKRES, LINC00980, PPD, Q10)
  • Promoter CMV
  • Tag / Fusion Protein
    • Myc (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSLIK-Hygro-Myc-hAgo2 was a gift from Zissimos Mourelatos (Addgene plasmid # 60234 ; http://n2t.net/addgene:60234 ; RRID:Addgene_60234)

  • For your References section:

    Precursor microRNA-programmed silencing complex assembly pathways in mammals. Liu X, Jin DY, McManus MT, Mourelatos Z. Mol Cell. 2012 May 25;46(4):507-17. doi: 10.1016/j.molcel.2012.03.010. Epub 2012 Apr 12. 10.1016/j.molcel.2012.03.010 PubMed 22503104
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