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Addgene

AAV:ITR-U6-sgRNA(backbone)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR Notes

Addgene Diagnostic Digest

This gel depicts the NEB 1kb ladder and a digestion of the plasmid AAV:ITR-U6-sgRNA(backbone)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR with the following enzyme: KpnI-HF.

We expect and see 2 bands at 5.4 and 1.2kb