pCI-FLAG-mTUT4(D1028A)
(Plasmid
#60220)
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PurposeMammalian expression of FLAG-tagged catalytic mutant of TUT4
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 60220 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCI
- Backbone size w/o insert (bp) 4000
- Total vector size (bp) 8900
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTUT4
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Alt nameZcchc11
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SpeciesM. musculus (mouse)
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Insert Size (bp)4900
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MutationD1028A
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Entrez GeneTut4 (a.k.a. 6030404K05Rik, 9230115F04Rik, PPAPD3, Tent, Tent3a, Zcchc11, mKIAA0191)
- Promoter CMV
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Tag
/ Fusion Protein
- FLAG (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BclI (destroyed during cloning)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCI-FLAG-mTUT4(D1028A) was a gift from Zissimos Mourelatos (Addgene plasmid # 60220 ; http://n2t.net/addgene:60220 ; RRID:Addgene_60220) -
For your References section:
A MicroRNA Precursor Surveillance System in Quality Control of MicroRNA Synthesis. Liu X, Zheng Q, Vrettos N, Maragkakis M, Alexiou P, Gregory BD, Mourelatos Z. Mol Cell. 2014 Aug 27. pii: S1097-2765(14)00610-8. doi: 10.1016/j.molcel.2014.07.017. 10.1016/j.molcel.2014.07.017 PubMed 25175028