Human-Dysferlin-v1
(Plasmid
#60217)
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PurposeHuman dysferlin exon 1 variant (accession #DQ267935) cDNA in pDONR221 plasmid which can be used as an entry vector for Invitrogen’s gateway system.
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 60217 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepDONR221
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 2571
- Total vector size (bp) 8860
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Vector typeGateway entry clone
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH10B
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameDysferlin
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SpeciesH. sapiens (human)
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Insert Size (bp)6289
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Entrez GeneDYSF (a.k.a. FER1L1, LGMD2B, LGMDR2, MMD1)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13pUC-fwd (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byThe human dysferlin insert was synthesized by DNA2.0
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Human-Dysferlin-v1 was a gift from Jain Foundation (Addgene plasmid # 60217 ; http://n2t.net/addgene:60217 ; RRID:Addgene_60217)
Map uploaded by the depositor.
