pcDNA_DiCre2_60.F2
(Plasmid #60210)

• Purpose: Expresses a fusion protein of FKBP12(F36V)-linker peptide 2- Cre(60-343)
• Depositing Lab: Jean-Paul Herman
• Publication: Herman's lab DiCre2-59.F2 plasmid (unpublished)

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Life Technologies
• Backbone size w/o insert (bp): 5428
• Total vector size (bp): 6655
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Cre recombinase
• Species: H. sapiens (human), Synthetic; Bacteriophage P1
• Insert Size (bp): 1244
• Mutation: FKBP12 has the F36V mutation; amino acids 60-343 of humanized version of Cre recombinase
• GenBank ID: NC_005856.1 NM_000801.4
• Entrez Gene: CD207 (a.k.a. CLEC4K)
• Promoter: CMV
• Tags / Fusion Proteins:
  • FKBP12(F36V) (N terminal on insert)
  • Nuclear localisation signal (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: PmeI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGHrev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that mutation numbering is relative to amino acid 2 of FKPB, mutation is F37V with respect to WT sequence.