pcDNA_DiCre_60.F2
(Plasmid
#60208)
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PurposeExpresses a fusion protein of FRB-linker peptide 2- Cre(60-343)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 60208 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1
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Backbone manufacturerLife Technologies
- Backbone size w/o insert (bp) 5428
- Total vector size (bp) 6619
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCre recombinase
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SpeciesH. sapiens (human), Synthetic; Bacteriophage P1
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Insert Size (bp)1199
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Mutationamino acids 2021-2112 of FRAP with a T2098L mutation; amino acids 60-343 of humanized version of Cre recombinase
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GenBank IDNC_005856.1 NM_004958.3
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Entrez GeneCD207 (a.k.a. CLEC4K)
- Promoter CMV
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Tags
/ Fusion Proteins
- FRAP (N terminal on insert)
- Nuclear localisation signal (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoR1 (not destroyed)
- 3′ cloning site PmeI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer BGHrev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA_DiCre_60.F2 was a gift from Jean-Paul Herman (Addgene plasmid # 60208 ; http://n2t.net/addgene:60208 ; RRID:Addgene_60208) -
For your References section:
Regulation of Cre recombinase by ligand-induced complementation of inactive fragments. Jullien N, Sampieri F, Enjalbert A, Herman JP. Nucleic Acids Res. 2003 Nov 1;31(21):e131. PubMed 14576331