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Addgene

pcDNA_DiCre_59.F2
(Plasmid #60207)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 60207 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Life Technologies
  • Backbone size w/o insert (bp) 5428
  • Total vector size (bp) 5914
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cre recombinase
  • Species
    H. sapiens (human), Synthetic; Bacteriophage P1
  • Insert Size (bp)
    515
  • Mutation
    amino acids 19-59 of the humanized version of Cre recombinase
  • GenBank ID
    NC_005856.1 NM_000801.4
  • Entrez Gene
    CD207 (a.k.a. CLEC4K)
  • Promoter CMV
  • Tags / Fusion Proteins
    • FKBP12 (N terminal on insert)
    • Nuclear localisation signal (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGHrev
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA_DiCre_59.F2 was a gift from Jean-Paul Herman (Addgene plasmid # 60207 ; http://n2t.net/addgene:60207 ; RRID:Addgene_60207)
  • For your References section:

    Regulation of Cre recombinase by ligand-induced complementation of inactive fragments. Jullien N, Sampieri F, Enjalbert A, Herman JP. Nucleic Acids Res. 2003 Nov 1;31(21):e131. PubMed 14576331