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Addgene

DYSF-Exon49-del
(Plasmid #60197)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 60197 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC57-Kan
  • Backbone size w/o insert (bp) 2363
  • Total vector size (bp) 8578
  • Vector type
    cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Dysferlin
  • Alt name
    DYSF
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    6215
  • Mutation
    Exon 49 has been deleted
  • Entrez Gene
    DYSF (a.k.a. FER1L1, LGMD2B, LGMDR2, MMD1)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer gagcagattgtactgagagtgcacg
  • 3′ sequencing primer M13pUC-rev
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The human dysferlin insert was synthesized by Genewiz

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    DYSF-Exon49-del was a gift from Jain Foundation (Addgene plasmid # 60197 ; http://n2t.net/addgene:60197 ; RRID:Addgene_60197)
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