pNB813
(Plasmid #60161)

• Purpose: Yeast codon-optimized yellow fluorescent protein (yEVenus) with PEST degron driven by SIC1 promoter.
• Depositing Lab: Nicolas Buchler
• Publication: Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14-07-1187.

Backbone

• Vector backbone: p405
• Backbone size w/o insert (bp): 6682
• Total vector size (bp): 7514
• Vector type: Yeast Expression
• Selectable markers: LEU2

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: yEVenus-PEST
• Alt name: Yeast codon-optimized yellow fluorescent protein fusion with PEST degron.
• Alt name: p405-SIC1pr-yEVenus-PEST
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 1257
• Promoter: SIC1pr
• Tag / Fusion Protein:
  • PEST degron (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: PacI (not destroyed)
• 3′ cloning site: AscI (not destroyed)
• 5′ sequencing primer: cactttatgcttccggctcctatgtt
• 3′ sequencing primer: CTGCCGGTAGAGGTGTGGTCAATAAG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry