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Addgene

pNB812
(Plasmid #60160)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 60160 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p405
  • Backbone size w/o insert (bp) 6682
  • Total vector size (bp) 7939
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    yEVenus-PEST
  • Alt name
    Yeast codon-optimized yellow fluorescent protein fusion with PEST degron.
  • Alt name
    p405-RNR1pr-yEVenus-PEST
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1257
  • Promoter RNR1pr
  • Tag / Fusion Protein
    • PEST degron (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PacI (not destroyed)
  • 3′ cloning site AscI (not destroyed)
  • 5′ sequencing primer cactttatgcttccggctcctatgtt
  • 3′ sequencing primer CTGCCGGTAGAGGTGTGGTCAATAAG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNB812 was a gift from Nicolas Buchler (Addgene plasmid # 60160 ; http://n2t.net/addgene:60160 ; RRID:Addgene_60160)

  • For your References section:

    "Measuring fast gene dynamics in single cells with timelapse luminescence microscopy". Mazo-Vargas A, Park H, Aydin M, Buchler NE. Mol Biol Cell. 2014 Sep 17. pii: mbc.E14-07-1187. 10.1091/mbc.E14-07-1187 PubMed 25232010
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