3HX4
(Plasmid #60137)

• Purpose: Bacterial expression for structure determination; may not be full ORF
• Depositing Lab: Cheryl Arrowsmith
• Publication: SGC Toronto Q1-Q3 2013 (unpublished)

Backbone

• Vector backbone: pET15-MHL
• Backbone manufacturer: SGC (Addgene plasmid # 26092)
• Backbone size w/o insert (bp): 7737
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TgCDPK4:MAC03F-A01:C208088
• Alt name: PDB IDs: 3HX4, 5DVR, 5DVT, 5DVU, 4IH8, 4IHP, 5JMS, 5JN2, 5W80, 5W8R, 5W91, 5W9E, 5W9R, 3KU2
• Alt name: TgCDPK4:MAC03D-DS-A01:C207456
• Alt name: CDPK1
• Species: Toxoplasma gondii
• Insert Size (bp): 1521
• Mutation: See comments
• Promoter: T7
• Tags / Fusion Proteins:
  • 6xHis (N terminal on backbone)
  • TEV cleavage site (N terminal on backbone)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 Terminal

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

For more information, please see:
PDB / SGC
3HX4 http://www.thesgc.org/structures/3hx4/
5DVR http://www.thesgc.org/structures/5dvr
5DVT http://www.thesgc.org/structures/5dvt
5DVU http://www.thesgc.org/structures/5DVU
4IH8 http://www.thesgc.org/structures/4IH8/
4IHP http://www.thesgc.org/structures/4IHP/
5JMS http://www.thesgc.org/structures/5JMS/
5JN2 http://www.thesgc.org/structures/5JN2/
5W80 http://www.thesgc.org/structures/5W80/
5W8R http://www.thesgc.org/structures/5W8R/
5W91 http://www.thesgc.org/structures/5W91/
5W9E http://www.thesgc.org/structures/5W9E/
5W9R http://www.thesgc.org/structures/5W9R/
3KU2 http://www.thesgc.org/structures/3KU2