pNB691
(Plasmid #60135)

• Purpose: Red beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by MET17 promoter.
• Depositing Lab: Nicolas Buchler
• Publication: Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14-07-1187.

Backbone

• Vector backbone: p406
• Backbone size w/o insert (bp): 5261
• Total vector size (bp): 7031
• Vector type: Yeast Expression
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: RdLuc
• Alt name: Red beetle luciferase
• Alt name: p406-MET17pr-NLS-RdLuc-ssrA
• Species: Synthetic
• Insert Size (bp): 1740
• Promoter: MET17pr
• Tags / Fusion Proteins:
  • Nuclear localization signal (NLS) (N terminal on insert)
  • ClpXP degron (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: PacI (not destroyed)
• 3′ cloning site: AscI (not destroyed)
• 5′ sequencing primer: cactttatgcttccggctcctatgtt
• 3′ sequencing primer: CTGCCGGTAGAGGTGTGGTCAATAAG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry