pAMPK alpha2 delta312X
(Plasmid #60127)

• Purpose: expresses constitutively active AMPKalpha2
• Depositing Lab: Morris Birnbaum
• Publication: Birnbaum lab plasmids (unpublished)

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Total vector size (bp): 7400
• Modifications to backbone: Modification of Dr. Carling's wildtype contruct, Addgene plasmid # 15991
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: AMPK alpha 2
• Species: R. norvegicus (rat)
• Insert Size (bp): 2000
• Mutation: Truncated at residue 312
• Entrez Gene: Prkaa2 (a.k.a. Ampk, Ampka2)
• Tag / Fusion Protein:
  • Myc (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGH rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The original plasmid was received from David Carling at the Imperial College School of Medicine in London in 1998
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments