pNB774
(Plasmid #60114)

• Purpose: Green beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA)
• Depositing Lab: Nicolas Buchler
• Publication: Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14-07-1187.

Backbone

• Vector backbone: pJ204
• Backbone manufacturer: DNA 2.0
• Backbone size w/o insert (bp): 2707
• Total vector size (bp): 4465
• Vector type: Unspecified

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: GrLuc
• Alt name: Green beetle luciferase
• Alt name: pJ204-NLS-GrLuc-ssrA
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 1731
• Tags / Fusion Proteins:
  • Nuclear localization signal (NLS) (N terminal on insert)
  • ClpXP degron (C terminal on insert)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry