2P0E
(Plasmid #60109)

• Purpose: Bacterial expression for structure determination; may not be full ORF
• Depositing Lab: Cheryl Arrowsmith
• Publication: SGC Toronto Q1-Q3 2013 (unpublished)

Backbone

• Vector backbone: pET28-LIC
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: C9orf95:C9orf95_dml-X01:C201158
• Alt name: PDB ID: 2P0E
• Alt name: C9orf95:HPC041-D03:C28049
• Alt name: NRK1
• Species: H. sapiens (human)
• Insert Size (bp): 564
• Mutation: See comments
• GenBank ID: BC001366 PDB ID: 2P0E
• Entrez Gene: NMRK1 (a.k.a. C9orf95, NRK1, bA235O14.2)
• Promoter: T7
• Tag / Fusion Protein:
  • 6xHis (N terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 Terminal

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

http://www.thesgc.org/structures/2P0E/