4JXT B
(Plasmid #60106)

• Purpose: Bacterial expression for structure determination; may not be full ORF
• Depositing Lab: Cheryl Arrowsmith
• Publication: SGC Toronto Q1-Q3 2013 (unpublished)

Backbone

• Vector backbone: pET15-MHL
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: RPRD1B:DCC020-G07:C222611
• Alt name: PDB ID: 4JXT, 4FLD, 4FU3, 4Q94, 4Q96
• Alt name: RPRD1B
• Species: H. sapiens (human)
• Insert Size (bp): 402
• Mutation: See comments
• GenBank ID: BC045623
• Entrez Gene: RPRD2 (a.k.a. HSPC099, KIAA0460)
• Promoter: T7
• Tag / Fusion Protein:
  • 6xHis (N terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 Terminal

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

http://www.rcsb.org/pdb/files/fasta.txt?structureIdList=4JXT
https://www.rcsb.org/structure/4Q94
https://www.rcsb.org/structure/4Q96

SGC links:
https://www.thesgc.org/structures/4JXT/
https://www.thesgc.org/structures/4FLD/
https://www.thesgc.org/structures/4FU3/
https://www.thesgc.org/structures/4Q94/
https://www.thesgc.org/structures/4Q96/