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Addgene

4MYJ
(Plasmid #60104)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 60104 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFBOH-MHL (Addgene #62304)
  • Vector type
    Baculovirus Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PF14_0346:MAC04G-A11:C228843
  • Alt name
    PDB IDs: 4MYJ, 8EM8
  • Alt name
    PfPKG, PP-PKG
  • Species
    Plasmodium falciparum
  • Insert Size (bp)
    2559
  • Mutation
    See comments
  • Promoter Polyhedrin
  • Tags / Fusion Proteins
    • 6xHis-TEV (N terminal on insert)
    • MHHHHHHSSGRENLYFQG (N terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer pFBOH-fwd (5'-CCGGATTATTCATACCGTCCCACCA-3')
  • 3′ sequencing primer pFBOH-rev (5'-CTGATTATGATCCTCTAGTACTTCT-3')
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    4MYJ was a gift from Cheryl Arrowsmith (Addgene plasmid # 60104 ; http://n2t.net/addgene:60104 ; RRID:Addgene_60104)
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