4MX2
(Plasmid #60102)

• Purpose: Bacterial expression for structure determination; may not be full ORF
• Depositing Lab: Cheryl Arrowsmith
• Publication: SGC Toronto Q1-Q3 2013 (unpublished)

Backbone

• Vector backbone: pET15-MHL
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: LdBPK_040440.1:MAC04A-B07:C226244
• Alt name: PDB ID:4MX2
• Alt name: LdASL
• Species: Synthetic; Leishmania donovani
• Insert Size (bp): 1365
• Mutation: See comments
• Promoter: T7
• Tag / Fusion Protein:
  • 6xHis (N terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 Terminal

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

http://www.thesgc.org/structures/4MX2/