4L59
(Plasmid #60087)

• Purpose: Bacterial expression for structure determination; may not be full ORF
• Depositing Lab: Cheryl Arrowsmith
• Publication: SGC Toronto Q1-Q3 2013 (unpublished)

Backbone

• Vector backbone: pET28-MHL
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: L3MBTL3:JMC057-A08:C223215
• Alt name: PDB ID: 4L59
• Alt name: L3MBTL3
• Species: H. sapiens (human)
• Insert Size (bp): 948
• Mutation: See comments
• GenBank ID: gi|55770869
• Entrez Gene: L3MBTL3 (a.k.a. MBT-1, MBT1)
• Promoter: T7
• Tag / Fusion Protein:
  • 6xHis (N terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 Terminal

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

http://www.thesgc.org/structures/4L59/