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Addgene

4IKP
(Plasmid #60080)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 60080 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pFBOH-MHL
  • Backbone manufacturer
    SGC (Addgene Plasmid #62304)
  • Vector type
    Baculovirus Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CARM1:JMC049-A06:C226836
  • Alt name
    PDB IDs: 4IKP, 6D2L, 6DVR, 5U4X
  • Alt name
    CARM1_21
  • Alt name
    CARM1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1032
  • Mutation
    See comments
  • GenBank ID
    gi|40288287 CARM1
  • Entrez Gene
    CARM1 (a.k.a. PRMT4)
  • Promoter polyhedrin
  • Tag / Fusion Protein
    • 6xHis (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer pFBOH-fwd (5'-CCGGATTATTCATACCGTCCCACCA-3')
  • 3′ sequencing primer pFBOH-rev (5'-CTGATTATGATCCTCTAGTACTTCT-3')
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    4IKP was a gift from Cheryl Arrowsmith (Addgene plasmid # 60080 ; http://n2t.net/addgene:60080 ; RRID:Addgene_60080)
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