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pSinRep5-181/25ic
(Plasmid #60078)

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This material is available to academics and nonprofits only. Availability may be limited outside the U.S. Please log in for more information.
Item Catalog # Description Quantity Price (USD)
Plasmid 60078 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pSinRep5
  • Backbone manufacturer
    made by Charles McGee
  • Backbone size w/o insert (bp) 1842
  • Total vector size (bp) 13905
  • Modifications to backbone
    Sindbis virus coding sequences removed. Only sequences necessary for replication and selection in E. coli maintained.
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Chikungunya virus strain 181/25 infectious clone
  • Alt name
    vaccine strain
  • Alt name
    181/25
  • Species
    Chikungunya virus strain 181/25
  • Insert Size (bp)
    12063
  • GenBank ID
    L37661.3
  • Promoter SP6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer SP6 forward primer
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSinRep5-181/25ic was a gift from Terence Dermody (Addgene plasmid # 60078)

  • For your References section:

    Reovirus cell entry requires functional microtubules. Mainou BA, Zamora PF, Ashbrook AW, Dorset DC, Kim KS, Dermody TS. MBio. 2013 Jul 2;4(4). pii: e00405-13. doi: 10.1128/mBio.00405-13. 10.1128/mBio.00405-13 PubMed 23820395
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