GFP-CYLD-STOP
(Plasmid
#60077)
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PurposeN-terminal GFP tagged CYLD with stop codon, driven by CMV promoter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 60077 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepENTR
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Backbone manufacturerLife Technologies
- Backbone size w/o insert (bp) 2600
- Total vector size (bp) 3000
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCylindromatosis
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SpeciesH. sapiens (human)
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Insert Size (bp)400
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Entrez GeneCYLD (a.k.a. BRSS, CDMT, CYLD1, CYLDI, EAC, FTDALS8, MFT, MFT1, SBS, TEM, USPL2)
- Promoter CMV
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Tag
/ Fusion Protein
- GFP (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CMVF (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GFP-CYLD-STOP was a gift from Stephen Elledge (Addgene plasmid # 60077 ; http://n2t.net/addgene:60077 ; RRID:Addgene_60077) -
For your References section:
The tumor suppressor CYLD regulates entry into mitosis. Stegmeier F, Sowa ME, Nalepa G, Gygi SP, Harper JW, Elledge SJ. Proc Natl Acad Sci U S A. 2007 May 22. 104(21):8869-74. 10.1073/pnas.0703268104 PubMed 17495026