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Addgene

pcDNA3-mDIS3-FLAG
(Plasmid #60046)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 60046 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone size w/o insert (bp) 5400
  • Total vector size (bp) 8300
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DIS3
  • Alt name
    RRP44
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2900
  • Entrez Gene
    Dis3 (a.k.a. 2810028N01Rik)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer Sp6
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-mDIS3-FLAG was a gift from Zissimos Mourelatos (Addgene plasmid # 60046 ; http://n2t.net/addgene:60046 ; RRID:Addgene_60046)

  • For your References section:

    A MicroRNA Precursor Surveillance System in Quality Control of MicroRNA Synthesis. Liu X, Zheng Q, Vrettos N, Maragkakis M, Alexiou P, Gregory BD, Mourelatos Z. Mol Cell. 2014 Aug 27. pii: S1097-2765(14)00610-8. doi: 10.1016/j.molcel.2014.07.017. 10.1016/j.molcel.2014.07.017 PubMed 25175028
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