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Addgene

pCI-FLAG-mRRP6
(Plasmid #60045)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 60045 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCI
  • Backbone size w/o insert (bp) 4000
  • Total vector size (bp) 6700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RRP6
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2700
  • Entrez Gene
    Exosc10 (a.k.a. RP23-331P21.1, PM-Scl, PM/Scl-100, Pmscl2, RRP6, p2, p3, p4)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Open Biosystems
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCI-FLAG-mRRP6 was a gift from Zissimos Mourelatos (Addgene plasmid # 60045 ; http://n2t.net/addgene:60045 ; RRID:Addgene_60045)

  • For your References section:

    A MicroRNA Precursor Surveillance System in Quality Control of MicroRNA Synthesis. Liu X, Zheng Q, Vrettos N, Maragkakis M, Alexiou P, Gregory BD, Mourelatos Z. Mol Cell. 2014 Aug 27. pii: S1097-2765(14)00610-8. doi: 10.1016/j.molcel.2014.07.017. 10.1016/j.molcel.2014.07.017 PubMed 25175028
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