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Mouse Nanog locus 3' probe
(Plasmid #60037)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 60037 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCR2.1
  • Backbone manufacturer
    invitrogene
  • Vector type
    Unspecified ; cloning

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Nanog
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    700
  • Entrez Gene
    Nanog (a.k.a. 2410002E02Rik, ENK, Stm1, ecat4)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The plasmid can be cut with BamH1 - EcoRV in order to get 0.7kb probe for Southern blot analysis. Digest genomic DNA with Sal1.

Please visit https://www.biorxiv.org/content/early/2014/08/23/008284 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Mouse Nanog locus 3' probe was a gift from Jacob Hanna (Addgene plasmid # 60037 ; http://n2t.net/addgene:60037 ; RRID:Addgene_60037)

  • For your References section:

    Transient acquisition of pluripotency during somatic cell transdifferentiation with iPSC reprogramming factors. Maza I, Caspi I, Zviran A, Chomsky E, Rais Y, Viukov S, Geula S, Buenrostro JD, Weinberger L, Krupalnik V, Hanna S, Zerbib M, Dutton JR, Greenleaf WJ, Massarwa R, Novershtern N, Hanna JH. Nat Biotechnol. 2015 Jun 22. doi: 10.1038/nbt.3270. 10.1038/nbt.3270 PubMed 26098448
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